Analytical Cytometry IV

Brno/South Moravia

June 23 – 26, 2007

The conference is under auspices of

Prof. RNDr. Václav Pačes, DrSc.

Chairman of Academy of Sciences of the Czech Republic

Conference Program

Saturday June the 23^rd2007
11.00 – 17.30	Bike Excursion (South Moravia region)
17:30	Registration Counter Opens
18.30 – 20.30	Opening Ceremony

Sunday June the 24^th 2007
Morning Session I (8.30 - 10.30) Plenary Lectures, Stem Cells Chairman: A. Kozubík
8.30 – 9.20	Cytomics and the Integration of Next-Generation Technologies	Robinson J. P.
9.20 – 10.10	Flow cytometry and cell proliferation: what we can do, or is propidium iodide staining enough?	Witkowski J.
10.10 – 10.30	Stemness: mechanisms controlling stem cell self-renewal and differentiation	Motlík J. et al.
Coffee Break (10.30 – 11.00)
Morning Session II (11.00 - 12.50) Plenary Lectures, Advanced Cytometry, Data Analyses Chairmen: J. Motlík, S. Kozubek
11.00 – 11.50	How to overcome trastuzumab resistance in ErbB2 targeted immunotherapy	Szöllösi J. et al.
11.50 – 12.10	Optimizing light through put in image cytometry	Kozubek M. et al.
12.10 – 12.30	Optimal detection setup in 9-colors polychromatic flow cytometry	Kalina T. et al.
12.30 – 12.50	Correlation structure of gene expression data	Pavlík T. et al.
Sunday June the 24^th 2007
Lunch (13.00 – 14.30)
Afternoon Session I (14.30 -16.15) Advanced Cytometry Chairmen: S. Kozubek, J. Vondráček
14.30 – 14.45	Fluorescent microscopy of living cells	Vařecha M. et al.
14.45 – 15.00	The role of cytoskeletal networks in plasmid DNA intracellular trafficking toward the cell nucleus and intranuclear movement of plasmid DNA	Ondřej V. et al.
15.00 – 15.15	Lamin a/c deficiency and nuclear structure	Galiová G. et al.
15.15 – 15.30	Nuclear topography of the c-myc gene and its transcript in HT-29 cells during enterocytic differentiation	Harničarová A. et al.
15.30 – 15.45	Deconvolution methods and their application in fluorescence microscopy	Kašík M.
15.45 – 16.00	cDNA microarrays in combination with both fluorescent in situ hybridization and immunostaining techniques	Stejskal S. et al.
16.00 – 16.15	Protein microarrays	Šimara P. et al.
Coffee break 16.15 - 16.45
Afternoon session II (16.45 – 18.30)
16.45 – 18.30	Poster Session
18.30 – 20.30	CSAC Meeting
20.30 – 24.00	Welcome Party

Monday June the 25^th 2007
Morning session III (8.30 – 10.30) Plenary Lectures, Plant Cytometry Chairman: J. Doležel
8.30 – 9.20	The Her2 Receptor-Tyrosine-Kinase in the Context of erbB-Receptors - Importance to Diagnosis and Therapy of Solid Tumors		Brockhoff G.
9.20 – 10.10	Incorporating flow and image cytometry into systems biology		Galbraith D.W.
10.10 – 10.30	Chromosome sorting using flow cytometry facilitates genome analysis in barley, rye and wheat		Doležel et al.
Coffee Break 10.30 - 11.00
Morning session IV (11.00 - 12.40) Chairmen: B. Říhová, J. Hofmanová
11.00 – 11.20	The role of an anti-apoptotic Mcl-1 protein in regulation of colon cancer cell sensitivity to TRAIL-induced apoptosis		Vaculová A. et al.
11.20 – 11.40	PML/RARa fusion oncoprotein, new mechanisms of gene deregulation in leukemia and aimed therapy		Falk M. et al.
11.40 – 12.00	Comparison of dendritic cells in healthy volunteers and subjects with monoclonal gammopathy and/or multiple myeloma		Kovářová L. et al.
12.00 – 12.20	Challenge for 8 color panels to improve sensitivity of minimal residual disease detection in childhood acute lymphoblastic leukemia. Evaluation of minimal residual disease and non leukemic background in the bone marrow during the therapy		Mejstříková E. et al.
12.20 – 12.40	Contribution of CD127 for human regulatory T cells immunophenotyping		Král V. et al.
Lunch 13.00 - 14.30
Monday June the 25th 2007
Afternoon Session III (14.30 -15.45) Cell Biology and Signaling Chairmen: M. Červinka, K. Souček
14.30 – 14.45	Importance of cellular lipid modulation for apoptotic and differentiation response of colon epithelial cells	Hofmanová J. et al.
14.45 – 15.00	Effect of hypoxia on chemoresistance and cell cycle changes of neuroblastoma cell lines	Hraběta J. et al.
15.00 – 15.15	Apoptosis and inhibition of gap-junctional intercellular communication induced by LA-12, a novel hydrophobic platinum(IV) complex	Turánek J et al.
15.15 – 15.30	Aberrant CD66c and leukemic cell signaling	Kanderová V. et al.
15.30 – 15.45	Efficient gene transfer into in-vitro cultures using the Amaxa nucleofection system and fluorescence-activated cell sorting of EGFP^+ cells	Borský M. et al.
Coffee Break 15.45 - 16.00
Afternoon Session IV (16.00 - 19.00)
16.00 – 17.00	Poster Session
17.00 – 19.00	Sponsor Presentations
20.00 – 24.00	Conference Dinner

Tuesday June the 26^th 2007
Morning session V (8.30 - 10.30) Stem Cells Chairmen: J. Motlík, P. Vodička
8.30 – 8.45	A new alternative in the isolation of porcine epidermal stem cells		Klíma J. et al.
8.45 – 9.00	Biodegradable porous hydrogels with biomimetic structure modification – cell culture study		Vodička P. et al.
9.00 – 9.15	Chondrogenic potential of the rabbit mesenchymal stem cells after allotransplantation into the physeal defect		Hlučilová J. et al.
9.15 – 9.30	Dental pulp stem cells: phenotypic changes according to culture conditions		Soukup T. et al.
Data Analyses Chairmen: L. Dušek, J. Jarkovský
9.30 – 9.45	Computational biology and its contribution to the data analysis in molecular biology and genetics		Dušek L. et al.
9.45 – 10.00	Multivariate statistical methods in analysis of immunological data: case study on patients with cvid and iga deficiencies		Jarkovský J. et al.
10.00 – 10.15	Technological, mamnagement and analytic background for multi-centric standardized collection of cytogenetic and molecular-genetic data in clinical registers: case study on camelia registry (chronic myelogenous leukemia)		Mužík J. et al.
10.15 – 10.30	Pitfalls and quests of DNA microarray data analysis: case study on patients with DLBCL lymphoma		Budinská E. et al.
Coffee Break 10.30 - 11.00
Morning session VII (11.00 - 12.45) Plant Cytometry Chairmen: J. Doležel, J. Suda
11.00 – 11.15	Flow cytometry as a powerful tool in plant conservation biology		Suda J. et al.
11.15 – 11.30	Allium oleraceum: polyploid complex of european geophytes		Šafářová L. et al.
11.30 – 11.45	GC content in plant genomes		Bureš P. et al.
Tuesday June the 26th 2007
11.45 – 12.00	Isolation of chromosome-specific genes by combining chromosome flow sorting and DNA hybridization techniques		Hobza R. et al.
Experimental oncology Chairman: M. Hajdúch
12.00 – 12.15	Macarpine - New supravital DNA probe		Slaninová I.et al.
12.15 – 12.30	Influence of sample purity on gene expression: different types of separation of B-chronic lymphocytic leukemia cells		Kotašková J. et al.
12.30 – 12.45	Multidrug resistance proteins and their significance in asthma bronchiale and nonspecific inflammatory bowel diseases		Džubák P. et al.
Lunch 13.00 - 14.30
Morning Session VI (8.30 -10.00) Clinical Cytometry Chairmen: T. Eckschlager, M. Klabusay
8.30 – 8.45	Human peripheral blood lymphocyte subsets as irradiation dose indicators – a flow cytometric study	Řeháková Z. et al.
8.45 – 9.00	Flow-cytometry analysis employment in estimation of reactive oxygen species production in human ejaculate samples	Novotný J. et al.
9.00 – 9.15	Multiplexed fluorescent bead array analysis of cytokines in septic patients	Průcha M. et al.
9.15 – 9.30	Expression of adhesion molecules on CD34^+ mobilized peripheral blood stem cells	Suková V. et al.
9.30 – 9.45	Indirect quantification of doxorubicin nuclear accumulation	Hovorka O. et al.
9.45 – 10.00	Defects of immunity - late effect of childhood cancer and its treatment?	Eckschlager et al.
Tuesday June the 26th 2007
Coffee Break 10.30 - 11.00
Morning Session VIII (11.00 - 12.30) Clinical Cytometry Chairmen: T. Kalina, M. Svoboda
11.00 – 11.15	Analysis of chimerism in patients after allogeneic bone marrow transplantation in cells of T, B, NK, monocyte and granulocyte lineages isolated by fluorescence-activated cell sorting	Klabusay M. et al.
11.15 – 11.30	Molecular taxonomy and predictive systems of human cancers based on gene expression profiling	Svoboda M.
11.30 – 11.45	Huntington disease and detection of steroid receptors	Molíková R. et al.
11.45 – 12.00	Detection of circulating tumor cells in colorectal cancer patients using real-time RT-PCR method	Kesselová M. et al.
12.00 – 12.15	Corelation of CEA and CA 19-9 serum levels with CEA expression and clinical characteristics of colorectal cancer patients	Srovnal J. et al.
12.15 – 12.30	Preparation of monoclonal antibody anti-lactosylceramide (LacCer) and their presence in lysosomal storage disorders	Koubek K. et al.
12.45 – 13.00	Declaration of „Contest of Young Scientists“ winners, Closing Remarks
Lunch 13.00 - 14.30