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Advanced immunophenotyping by flow cytometry

Flow cytometry satellite workshop ESID-ISAC-CSAC in Prague November 1-2 2014

ESID (European Society of Immunodeficiency)

ISAC (International Society for Advancements of Cytometry)

CSAC (Czech Society for Analytical Cytology)

Presentations for download:

Tomas Kalina - Instrument set-up, background, panel

Enrico Lugli - Practical Multicolor Flow Cytometry

Enrico Lugli - Data Presentation

Tomas Kalina - Flow Resources


TIMETABLE:

Saturday, November 1, 2014
13.15
Meeting at the registration desk of the 16th Biennial Meeting of the European Society for Immunodeficiencies (ESID 2014) – departure for lunch
13.30
Lunch in the restaurant “Kandelabr”, Štětkova 1638/18, Prague 4 | See the map
14.30 – 15.30
Sightseeing walk of Prague which will finish at the workshop venue (Emauzy abbey)
15.30 – 19.00
Scientific program
19.00       
Departure to workshop dinner | Novoměstský pivovar, Vodičkova 20, Prague 1 – see the map

Sunday, November 2, 2014
9.00 – 12.30   
Scientific program

PROGRAM (includes all maps) - download

Workshop lead by ISAC Scholars:

  • Tomas Kalina, Prague, Czech Republic (Bio)
  • Enrico Lugli, Milan, Italy (Bio)

Flow cytometry is a premier method for immune system interrogation that is undergoing a revolutionary increase in its potential (polychromatic, rare cell analysis, antigen specific assays). It is of importance that flow cytometry is used appropriately and that the data provided are of high quality. It is becoming more complicated with the increased number of parameters measured (8 or more) and with large data sets (large patient series, inter-laboratory collaborations, high cell number acquisition for rare cell detection). Educational workshop is jointly organized by ESID and cytometry societies ISAC and CSAC.

Workshop is organized as a sattelite immediatelly after conclusion of ESID 2014 biennal meeting held in Prague http://esid.org/

What can you expect:
Get a guided walk from ESID congress venue through mysterious Vysehrad fortress down to Emmaus Abbey to stretch your leggs and entertain your mind, enjoy advanced flow cytometry workshop at Emmaus Abbey. Learn how to generate robust mutliparametric data, how to analyze and interpret it.

Expected participants:

Immunologists (PhD students, post-docs, junior researchers) with a working knowledge of flow cytometry who wish to master challenges of more colors, reproducibility and robustnes of flow assays.

Participation fee 45   

Social diner for participants is included.


Registration is closed since the maximum capacity of the workshop has been reached. 

 

PARTNERS

                    

 

LOCATION

 

The workshop will be held in the beautiful city of Prague, capital of the Czech Republic, which offers its visitors a breathtaking historical environment.

Virtual tours: Official Website City of Prague, CzechSite Travel Guide, Prague Information Service

 

Workshop venue

The Emauzy Abbey

Vyšehradská 49/320

128 00  Prague 2

Czech Republic

Google map

 

The venue is situated close to the ESID Meeting venue (Prague Congress Centre) – see the map (by public transport)

 

The monastery was founded by Charles IV in 1347. Such personalities as Jan Hus, Jerome of Prague etc. were among the students. A number of illuminated literal jewels were created here, among others a so-called Reims Gospel-book and the first complete translation of Jerome’s Vulgata. The monastery together with the church was solemnly consecrated by archbishop Ocko from Vlasim in the presence of Charles IV on 29 March 1372. The wall paintings rank among the most precious relics of the Czech Gothic. In the 16th century abbot Matthew Benesovsky transformed the area into a tavern with musicians and a skittle-ground for entertainment; in the early 17th century the astronomer Kepler lived there for a few years.

In 1712 both the monastery and the church were renovated in the Baroque style, the high roof was replaced by a tripartite one, and two low frontal bulbous towers of the church were built.  After 1880 the monastery as well as the church were restored and altered to the so-called Beuron style.

The church was destroyed during an American air-raid in Prague on 14 February 1945. A modern structure designed by F. M. Černý was later installed: an asymmetric 32 meter high shell structure with a 40 cm thick construction of white concrete.

 

Public transport

Full information about the public transport is available at www.dpp.cz/en

All public transport runs between 5am- and 12am, everyday, reasonably often (metro every 5-15 min., buses max 20 min.)

 

How to get to the workshop venue

From the Airport:

 

By public transport:

- buy a ticket from a vending machine for CZK 32,- (90 min.)

- take bus No. 100 from the airport to the final stop Zličín

- take metro B to Karlovo náměstí station (  ̴20 minutes)

- walk five minutes according to the map

 

Alternatively

- buy a ticket from a vending machine for CZK 32,-

- take bus No. 119 from the airport to the final stop Dejvická

- take metro A to Mustek station (4 stops)

- change to line B (direction Zličín) to the Karlovo náměstí station (2 stops). The workshop venue is located within 300 m distance from the Karlovo náměstí station.

Airport minibus CEDAZ take passengers to the centre (more information on www.cedaz.cz).

Taxi (recommended company is AAA, phone 14014), price to the Emauzy Abbey should be around CZK 500,-.

 

From the Main Railway Station (Hlavní nádraží):

by metro:

Take the line C (direction Letňany) to the FLORENC station (1 stop) and there change to line B (direction Zličín) to the Karlovo náměstí station (4 stops). The workshop venue is located within 300 m distance from the Karlovo náměstí station.

Before you enter the metro you have to buy a CZK 24,- ticket from the vending machines or at a newsagent.

 

From the Holesovice Railway Station (Nádraží Holešovice):

by metro:

Take line C (direction Háje) to the FLORENC station (2 stops) and there change to line B (direction Zličín) to the Karlovo náměstí station (4 stops). The workshop venue is located within 300 m distance from the Karlovo náměstí station.

Before you enter the metro you have to buy a CZK 32,- ticket from the vending machines or at a newsagent.

 

Accommodation                                             

Accommodation options (in the surrounding area):

Accommodation reservation is not included in the registration form.

 

Best Western City Hotel Moran
Na Morání 15, Prague 2

Website (contact + reservation)               

Map

- 100 m from the workshop venue

- prices from 65 EUR

 

Hotel Park Inn Prague

Svobodova 1961/1, Prague 2

Website (contact + reservation)               

Map

- 650 m from the workshop venue (map)

- prices from 65 EUR

 

Novoměstský Hotel

Řeznická 4, Prague 1

Website (contact + reservation)               

Map                             

- 850 m from the workshop venue (map)

- prices from 45 EUR

 

Green Garden Hotel

Fügnerovo náměstí 1865/4, 120 00 Prague 2

Website (contact + reservation)               

Map                             

- 1100 m from the workshop venue (map) or 18 min. by tram (more info)

- prices from 85 EUR

 

 

Entertainment, sights

Much online info on Prague exists, one of the starting points can be www.prague.cz

 

Prices, money

Rate: EUR 1 is   ̴CZK 28,-

Banks are better than the tourist cash exchange outlets, but they are only opened from 9am – 5pm and not during the weekend.

Any convertible currency is accepted. Travelers cheques are accepted as well.

Prices largely depend much on where you go.

One can have a dinner for about CZK 200-400,-, beer for CZK 20-40,-, coffee for CZK 30-50,-

All major credit cards are accepted in department stores and tourist shops, not always in restaurants.

A wide network of ATM cash dispensers exist.

 

 
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