2018 EMBL Hands-on Flow Course

04/06 - 08/06/2018 EMBL Heidelberg, Germany https://www.embl.de/training/events/2018/CYT18-01/ 2018-06-04 00:00 2018-06-08 00:00 Europe/Prague 2018 EMBL Hands-on Flow Course EMBL Heidelberg, Germany

Course Overview

Flow Cytometry is one of the most powerful single cell analysis methods used throughout the life sciences and medicine. Utilising Flow Cytometry we can identify and quantify many different parameters from heterogeneous samples, analyse the corresponding state of proliferation or simply detect the expression of fluorescent reporters. In line with the suggestions from our course in 2017, we will significantly enhance the number of analysis sessions together with our partners from FlowJo. We will cover the theory of Flow Cytometry and corroborate this information intensively with the practical issues of the most frequent assays as part of our practical course curriculum. 

The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of Flow Cytometry is of advantage, but not strictly a must.

In-depth principles of Flow Cytometry
Quality assessment and instrument validation
Principles and guidelines for experimental design, especially for multi-color Flow Cytometry
Staining of tissues with cell surface antigens
Staining cell cycle profiles in fixed and live cells
Assessing proliferation and cell division
The beauty and burdens of fluorescence proteins in Flow Cytometry
Detailed multi-sessions on analysing data from Flow Cytometers in cooperation with FlowJo
If requested, exploration of very-high parametric Flow Cytometry data highlighting important experimental design and analysis issues with real data from collaborating laboratories.
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